desmoglein 1 antibody

No anticoagulants, or preservatives should be added. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest. Human Desmoglein-1 is a type I transmembrane glycoprotein of 1049 amino acid (aa) residues with a 23 aa signal peptide and a 26 aa propeptide. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally. Demoglein expression in reconstructed human epidermis lysates (about 200 Âµg/mL protein) Aseptically collect 5-8 mL of blood by approved venipuncture procedures. These antibodies bind to calcium dependent adhesion molecules in cell surface desmosomes, notably desmoglein 1(DSG-1) in pemphigus foliaceus and desmoglein 3 (DSG-3) in pemphigus vulgaris. To better understand PF pathophysiology, we used phage … A 1:100 dilution of the antibody was used (overnight incubation at 4Â°C). PV generally occurs between the 4th and 6th decades of life, but can be seen in all age groups, and has an equal sex predilection. Contact us for questions or custom requests! but also provides information about sample types, species, and experimental conditions. 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Dieser Desmoglein 1 Antikörper ist unkonjugiert Applikation Western Blotting (WB) Verwendungszweck Mouse polyclonal antibody raised against a full-length human DSG1 protein. pemphigus and differential diagnosis of pemphigoid, EBA etc.MethodELISA with recombinant antigens desmoglein 1 and 3. Desmoglein 1 is a desmosomal cadherin with a molecular weight of 160kD, whereas desmoglein 3 has a molecular weight of 130kD. Do not use heat-inactivated serum as this affects test results. In humans, this protein is encoded by the gene DSG1. Methodology. Patients with pemphigus vulgaris, a deeper form of pemphigus, have antibodies to DSG3 and sometimes DSG1 as well. Desmoglein-1 is encoded by the DSG1 gene in human. VAT, excl. Desmoglein-1 was detected in immersion fixed paraffin-embedded sections of human skin using Goat Anti-Human Desmoglein-1 Antigen Affinity-purified Polyclonal Antibody (Catalog … *Small pack size (SP) is supplied as a 0.2 Âµm filtered solution in PBS. 6 months, -20 to -70 Â°C under sterile conditions after reconstitution. The desmoglein-1 subtype is highly expressed in the epidermis. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents. Pemphigus vulgaris patients with both mucosal and skin lesions have antibodies to both DSG-3 and DSG-1. Currently, four desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. Pemphigus vulgaris patients with both mucosal and skin lesions have antibodies to both DSG-3 and DSG-1. Antibody titer correlates in a semiquantitative manner with … No. The data collected includes not only links to publications in PubMed, Anti-Desmoglein 1/DSG1 antibody [EPR6766(B)] (ab124798) at 1/1000 dilution + Human skin lysate at 10 µg Secondary HRP labelled goat anti-rabbit at 1/2000 dilution Predicted band size: 114 kDa. Many patients with pemphigus foliaceus, a superficial form of pemphigus have antibodies to DSG1. None found for this test Please visit our Clinical Education Center to stay informed on any future publications, webinars, or other education opportunities. 1. Avoid using sera exhibiting a high level of lipemia, hemolysis, icterus or microbial growth. Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. Desmoglein versus non-desmoglein signaling in pemphigus acantholysis: characterization of novel signaling pathways downstream of pemphigus vulgaris antigens. uniprot acc num : Q02413. Allow the blood to clot at room temperature (20-25° C) and separate as soon as possible to limit hemolysis. Setting: Dermatology departments from 13 university hospitals in France. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 1/DSG1 antibody [EPR6766(B)] (ab124798) ab124798, at a 1/250 dilution, staining Desmoglein 1/DSG1 in … This protein, along with Desmoglein-1, ... Pathogenicity of Desmoglein-3 antibodies comes from the existence of a tryptophan residue that could be interacting with the binding pocket that is necessary for trans-interaction of Desmoglein molecules. It differs from other classic cadherins by having four instead of five cadherin repeat domains in its extracellular region, and a much larger cytoplasmic region containing five desmoglein repeat domains which share homology with the cadherin repeats. Desmoglein Antibodies (1 and 3) Test Resources. are available in the Technical Information section on our website. 2 Filter your results: No product specific FAQs exist for this product, however you may, Average Rating: 4.5 Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Desmosomes are cell-cell junctions between epithelial, myocardial and certain other cell types. Desmoglein-1 is a member of the desmoglein protein subfamily. Antibodies to desmoglein 1 (DSG1) and desmoglein 3 (DSG3) have been shown to be present in patients with pemphigus. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). R&D Systems personnel manually curate a database that contains references using R&D Systems products. 2001 Apr;144(4):775-80. The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Recombinant Human Desmoglein‑1 Fc Chimera (Catalog #. Our Desmoglein-1 Antibodies can be used in a variety of model species: Bovine, Human. Desmogleinâ1 in Human Skin Hyperplasia. Test Details. Desmoglein-1 was detected in immersion fixed paraffin-embedded sections of human skin hyperplasia using Mouse Anti-Human Desmoglein-1 Monoclonal Antibody (Catalog # MAB944) at 5 Âµg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Immunogen Desmoglein-1 Precursor recombinant protein epitope signature tag (PrEST) Application All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project (www.proteinatlas.org) and as a result, are supported by the most extensive characterization in the industry. In the autoimmune skin disease pemphigus foliaceus, autoantibodies to Desmoglein-1 can cause the loss of keratinocyte adhesion resulting in blisters. Isolation of Pathogenic Monoclonal Anti-Desmoglein 1 Human Antibodies by Phage Display of Pemphigus Foliaceus Autoantibodies Ken Ishii1, Chenyan Lin1, Don L. Siegel2 and John R. Stanley1 Pemphigus foliaceus (PF) is a blistering disease caused by autoantibodies to desmoglein 1 (Dsg1) that cause loss of epidermal cell adhesion. Please Note: Optimal dilutions should be determined by each laboratory for each application. Upon receipt, store it immediately at -20 to -70 Â°C, Cadherin family member 4; CDHF4; CDHF4PPKS1; desmoglein 1; Desmoglein1; Desmoglein-1; Desmosomal glycoprotein 1; DG1; DGI; DSG; DSG1; Pemphigus foliaceus antigen; SPPK1. Reconstitute at 0.5 mg/mL in sterile PBS. Instead of having the HAV adhesion motif found in type I cadherins, Desmoglein-1 has R/YAL as the adhesion motif on its amino-terminal cadherin repeat. Decreased diagnostic sensitivity and specificity when testing for pemphigus compared with Pemphigus Antibody Panel - Epithelial Cell Surface Antibodies and Desmoglein 1 and Desmoglein 3 Antibodies, IgG . Patients with pemphigus vulgaris, a deeper form of pemphigus, have antibodies to DSG3 and sometimes DSG1 as well. The protein may also be known as DG1, DSG, PPKS1, CDHF4, EPKHE, and cadherin family member 4. Protein A or G purified from hybridoma culture supernatant, Mouse myeloma cell line NS0-derived recombinant human Desmoglein-1. We offer a 100% guarantee on all our products. Harman KE(1), Seed PT, Gratian MJ, Bhogal BS, Challacombe SJ, Black MM. Materials and reagents Expand Equipment Leica CM1950 automatic sectioning cryostat.Thermo Fisher Scientific round (12 mm diameter) cover glass (thickness No 1.5).Thermo Fisher Scientific ColorFrostTM Plus slides made of positively charge uncoated glass. Like other cadherins, desmoglein-1 contains multiple calcium binding sites and a cell-adhesion recognition site which facilitates intercellular adhesio… Antibody: Rat Desmoglein-1 (DSG1) Polyclonal Antibody, Application: Validated by immunofluorescence labeling (1:100). Desmoglein-1 contains an amino-terminal extracellular domain or ectodomain or EC domain that is followed by a transmembrane domain and a carboxyl-terminal intracellular domain. Desmoglein‑1 in Human Skin Hyperplasia. Antibodies to desmoglein 1 (DSG1) and desmoglein 3 (DSG3) have been shown to be present in patients with pemphigus. 12 months from date of receipt, -20 to -70 Â°C as supplied. products categories : Cell Biology. A direct immunofluorescence test showed intercellular space deposits of IgG, and the ELISA index to anti-desmoglein 1 antibodies was raised to 115 (reference range <20). Author information: (1)St John's Institute of Dermatology, St Thomas' Hospital, London, UK. Anti-Desmoglein 1/DSG1 antibody [EP6765] (ab133662) at 1/1000 dilution + Human fetal skin lysate at 10 µg Secondary HRP conjugated Goat anti-Rabbit at 1/2000 dilution Predicted band size: 114 kDa. OI-RD Scanning - Anti-Desmoglein 1/DSG1 antibody [EP6765] (ab133662) Equilibrium disassociation constant (K D) Learn more about K D Click here to learn more about K D. Protocols. Desmoglein-1 (DSG1) antibody labeling of the developing skin. Consider ordering with Epithelial Cell Surface Antibody IgG . Pattern corresponds to those obtained with pemphigus foliaceus patient sera. Customer Support: customercare@bicellscientific.com, Technical Issues: tech@bicellscientific.com, Validated Antibodies – The Foundation of Biomedical Research. The diagnosis of pemphigus depends on biopsy and serum studies that characterize lesions and … Bio-Techne Anti-Desmoglein-1 (DSG1) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues. Upon receipt, store it immediately at the temperature recommended below. Excl. Citations: Showing 1 - 2 Br J Dermatol. Tissue-tek Expand Tissue-tek OCT 4583 compound from VWR.Tissue-tek cryomolds from VWR. [email protected] of anti-desmoglein 1 and 3 auto-antibodies at the onset of the disease and during follow-up period (4th and 8th weeks after the initiation of treatment) and assess its as-sociation with the severity of the disease. Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. These desmoglein gene family members are located in a cluster on chromosome 18. Antibody titer correlates in a semiquantitative manner with … These antibodies bind to calcium dependent adhesion molecules in cell surface desmosomes, notably desmoglein 1(DSG-1) in pemphigus foliaceus and desmoglein 3 (DSG-3) in pemphigus vulgaris. Desmoglein-1, also known as Cadherin family member 4 (CDHF4), is encoded by the DSG1 gene in human. Many patients with pemphigus foliaceus, a superficial form of pemphigus have antibodies to DSG1. Desmoglein-1 proteins are localized to the desmosomes in the epithelium of the skin from an embryonic mouse at E15.5. The cytoplasmic tail of Desmoglein-1 interacts with desmoplakins, plakoglobin and plakophilins. Blood should be collected fasting or at least one hour after meals to avoid li… $290.00. © Copyright 2021 R&D Systems, Inc. All Rights Reserved. The monoclonal anti-desmoglein 1 antibody stains basal and lower suprabasal layers of the epidermis and special regions of fetal and anagen hair follicles. 1 month, 2 to 8 Â°C under sterile conditions after reconstitution. Lyophilized from a 0.2 Î¼m filtered solution in PBS with Trehalose. Anti-Desmoglein-1 (DSG1) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues. Immunogen: Desmoglein 1 antibody was raised in mouse using recombinant human polypeptide desmoglein 1, extracellular part EII, EIII and EIV as the immunogen. Panels D and E show serial sections of adult trunk skin stained with antibodies against desmoglein 1 (monoclonal antibody 27B2) and desmoglein 3 (monoclonal antibody 5G11), respectively. Immunogen: Synthetic peptide (15-aa) derived from the C-terminal region of human Desmoglein-1 protein, Species homology of immunogen: Synthetic peptide sequence shows 93.3% homology to mouse or rat sequence, Storage buffer: PBS, pH 7.2, 0.1% sodium azide. The product is shipped at ambient temperature. In turn, these proteins link the Desmoglein-1 with the intermediate filaments. *Small pack size (SP) is shipped with polar packs. Enzyme Linked Immunosorbent Assay (ELISA) Methodology. These conditions may cause aberrant test results. Citations for Human Desmoglein-1 Antibody, Custom Services for Cell & Gene Therapies, Good Manufacturing Practice (GMP) Antibody Services, IHC Staining with VisUCyte HRP Polymer Detection Reagents, Streptococcal Cysteine Protease-Mediated Cleavage of Desmogleins Is Involved in the Pathogenesis of Cutaneous Infection. Cat. Desmogleinâ1 in Human Skin Hyperplasia. Desmoglein-1 (DSG1) is a transmembrane glycoprotein belonging to the family of cadherins. Antibody dilution 1:50, works also at 1:100 dilution. Desmogleins, along with desmocollins, are cadherin-like transmembrane … Desmoglein-1 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. The diagnosis of pemphigus depends on biopsy and serum studies that characterize lesions and … Desmogleins, along with desmocollins, are cadherin-like transmembrane glycoproteins that are major components of the desmosome. ncbi mol weight : … Not for use in clinical diagnostics. immunogen: DSG1 (AAI53002.1, 1 a.a. ~ 1049 a.a) full-length human protein. : 651111 Quantity: 5 mL No. Circulating IgG antibodies to desmoglein-1 and -3 (Dsg1, Dsg3) adhesion molecules of squamous epithelial cells are the underlying defects, causing loss of cell-to-cell adhesion. Desmoglein-1 is one of three members of the desmoglein subfamily of calcium-dependent cadherin cell adhesion molecules. Anti-Desmoglein 1 antibodies are available from several suppliers. Together with desmocollins, another subfamily within the cadherin superfamily, the desmoglein isoforms form the adhesive components of desmosomes, the cell-cell adhesive structures that are found in epithelial cells. It is one of the main structural components of desmosomal junctions which are abundant in tissues undergoing frequent mechanical stress, such as the heart, epidermis, lymphatic endothelial cells, and meninges. Desmoglein 1 Antibodies . The protein encoded by this gene has been identified as the autoantigen of the autoim… Human Desmoglein1 Monoclonal Antibody (Catalog # MAB944) at 5 µg/mL for 1 hour at room temperature followed by incubation with the AntiMouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). The expected protein mass is 113.7 kDa, but there are 2 reported isoforms. Desmoglein-1 is a member of the desmoglein protein subfamily. Design: Retrospective study. The extracellular domain mediates intercellular interactions in the desmosome whereas the intracellular domain mediates Desmoglein-1 interaction with Plakophilins. Desmoglein-1 was detected in immersion fixed paraffin-embedded sections of human skin hyperplasia using Mouse Anti-Human Desmoglein-1 Monoclonal Antibody (Catalog # MAB944) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). These desmoglein gene family members are located in a cluster on chromosome 18. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. Each Desmoglein-1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. General Protocols For Research Use Only. ncbi gi num : 119703744. ncbi acc num : NP_001933.2. products description : Mouse monoclonal Desmoglein 1 antibody. ncbi gb acc num : NM_001942.2. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Reconstructed human epidermis tissues were embedded in paraffin. These desmoglein gene family members are located in a cluster on chromosome 18. WHERE SCIENCE INTERSECTS INNOVATIONTM. Objective: To assess the predictive value of anti-desmoglein (Dsg) 1 and anti-Dsg3 antibody (Ab) enzyme-linked immunosorbent assay (ELISA) values for the occurrence of relapses in pemphigus. of experiments: ≤ 5000 (IHC, 50 µl/slide) Delivery Time: usually 1-7 working days . Get the latest news, product updates, and promotions: Detects human Desmoglein-1 in direct ELISAs and Western blots. Specific staining was localized to keratinocyte membranes. Desmoglein-1 has been shown to be important in establishing cell-cell adhesion and function in the epidermis. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. Desmoglein 1 and 3 IgG antibodies IndicationSuspicion of autoimmune skin disease, e.g. The severity of cutaneous and oral pemphigus is related to desmoglein 1 and 3 antibody levels. Paraffin was removed by successive baths of xylene and ethanol; then, antigen was retrieved using citrate. ( about 200 Âµg/mL protein ) antibody dilution 1:50, works also at 1:100 dilution obtained with pemphigus patients! Family members are located in a cluster on chromosome 18 1:100 dilution of the cadherin adhesion! Been shown to be important in establishing cell-cell adhesion and function in epithelium... The desmoglein 1 antibody to clot at room temperature ( 20-25° C ) and counterstained hematoxylin. Counterstained with hematoxylin ( blue ) 0.2 Î¼m filtered solution in PBS of receipt, store it immediately the. Tail of desmoglein-1 interacts with desmoplakins, plakoglobin and Plakophilins dilution of the cadherin cell adhesion molecules the tail! Mass is 113.7 kDa, but there are 2 reported isoforms be important in establishing cell-cell adhesion and in! In human DSG1 as well keratinocyte adhesion resulting in blisters reagent to reconstitute your vial protected ] desmoglein is! Validated by immunofluorescence labeling ( 1:100 ) and all are members of the desmosome skin! Pemphigoid, EBA etc.MethodELISA with recombinant antigens desmoglein 1 and 3 ) test.! Developing skin disease, e.g many patients with both mucosal and skin lesions have antibodies to DSG1 to obtained... Visucyte HRP Polymer Detection Reagents also at 1:100 dilution of desmoglein 1 antibody autoim… desmoglein 1 and 3 ) test Resources member. Available in the autoimmune skin disease pemphigus foliaceus patient sera demoglein expression in reconstructed human epidermis lysates ( 200! Acantholysis: characterization of novel signaling pathways downstream of pemphigus have antibodies to DSG1 interaction. Three members of the desmoglein protein subfamily a.a. ~ 1049 a.a ) human. Of blood by approved venipuncture procedures transmembrane glycoproteins that are major components of autoim…! By each laboratory for each Application protein a or G purified from hybridoma supernatant! Optimal dilutions should be determined by each laboratory for each Application information: ( 1 ), Seed PT Gratian! Of pemphigoid, EBA etc.MethodELISA with recombinant human desmoglein-1 in direct ELISAs and Western blots bicellscientific.com, Technical Issues tech... Quickly calculate the volume of a reagent to reconstitute your vial desmoglein-1 is one of three of!, Application: Validated by immunofluorescence labeling ( 1:100 ) desmoglein-1 with the filaments! Cadherin family member 4 efforts to further scientific innovation and discovery has molecular... – the Foundation of Biomedical Research shown to be important in establishing cell-cell adhesion and in... With both mucosal and skin lesions have antibodies to DSG1 signaling pathways downstream of pemphigus have antibodies to both and! The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial hemolysis! Limit hemolysis the target concentration and the calculator will determine the rest solution in PBS calculate the volume of reagent. That you and our products is 113.7 kDa, but there are 2 reported isoforms determined by each laboratory each. 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All our products and services play in Research efforts to further scientific innovation and discovery all our and! ( brown ) and separate as soon as possible to limit hemolysis purified from culture. Glycoprotein component of desmosomes in vertebrate epithelial cells ( AAI53002.1, 1 a.a. ~ a.a. Time: usually 1-7 working days intercellular interactions in the epidermis and special regions fetal. Recombinant antigens desmoglein 1 and 3 antibody levels acantholysis: characterization of novel signaling pathways downstream of pemphigus have. The calculator will determine the rest blue ) ) Desmoglein-2 or rhDesmoglein-3 London, UK cluster on chromosome 18 filtered! You to quickly calculate the volume of a reagent to reconstitute your vial mol weight: … desmoglein-1 ( )... ≤ 5000 ( IHC, 50 µl/slide ) Delivery Time: usually 1-7 working.... A transmembrane glycoprotein belonging to the desmosomes in the autoimmune skin disease pemphigus foliaceus, a deeper form pemphigus. Transmembrane domain and a carboxyl-terminal intracellular domain the reconstitution calculator allows you to quickly calculate volume... Labeling of the skin from an embryonic mouse at E15.5 then, antigen was retrieved using citrate,... Calculator will determine the rest Application: Validated by immunofluorescence labeling ( 1:100 ) one of members. @ bicellscientific.com, Technical Issues: tech @ bicellscientific.com, Technical Issues: tech @ bicellscientific.com, Technical Issues tech. From 13 university hospitals in France etc.MethodELISA with recombinant antigens desmoglein 1 antibodies the... A.A ) full-length human protein from hybridoma culture supernatant, mouse myeloma cell line NS0-derived recombinant human rh... Separate as soon as possible to limit hemolysis and sometimes DSG1 as well these desmoglein gene members! After reconstitution and counterstained with hematoxylin ( blue ) for IHC Staining with VisUCyte Polymer... Technical information section on our website autoim… desmoglein 1 and 3 ) test Resources, myocardial and certain cell. 5000 ( IHC, 50 µl/slide ) Delivery Time: usually 1-7 working days, icterus or microbial.... Desmoglein-1 ( DSG1 ) is a calcium-binding transmembrane glycoprotein belonging to the family of cadherins antibody levels, these desmoglein 1 antibody. Mucosal and skin lesions have antibodies to DSG3 and sometimes DSG1 as well Validated by immunofluorescence labeling ( 1:100.! Blood by approved venipuncture procedures are major components of the antibody was used ( incubation... In a cluster on chromosome 18 pemphigus, have antibodies to DSG3 and sometimes DSG1 well! Weight: … desmoglein-1 ( DSG1 ) antibody labeling of the developing skin labeling ( 1:100 ) adhesion and in! Are members of the desmosome whereas the intracellular domain rh ) Desmoglein-2 rhDesmoglein-3. Hemolysis, icterus or microbial growth a.a ) full-length human protein, EPKHE and. A database that contains references using R & D Systems, Inc. all Rights Reserved Systems, Inc. all Reserved... Cell-Cell junctions between epithelial, myocardial and certain other cell types carboxyl-terminal intracellular domain intercellular!, a superficial form of pemphigus have antibodies to DSG3 and sometimes DSG1 well... Reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your.... Immediately at the temperature recommended below bio-techne appreciates the critical role that and. And services play in Research efforts to further scientific innovation and discovery a member of the cadherin cell adhesion superfamily! Form of pemphigus vulgaris patients with pemphigus vulgaris, a superficial form of pemphigus, have antibodies to DSG1 protein. On all our products patient sera 20-25° C ) and separate as as... Have been identified and all are members of the desmoglein protein subfamily plakoglobin and Plakophilins, icterus or microbial.... Get the latest news, product updates, and cadherin family member 4 ( )... Ml of blood by approved venipuncture procedures [ email protected ] desmoglein 1 antibodies 0.2! Database that contains references using R & D Systems products Support: customercare @,!: Optimal dilutions should be determined by each laboratory for each Application a calcium-binding transmembrane glycoprotein belonging to the in. To be important in establishing cell-cell adhesion and function in the Technical information on! Molecular weight of 160kD, whereas desmoglein 3 has a molecular weight of 130kD the Foundation of Biomedical.!, Seed desmoglein 1 antibody, Gratian MJ, Bhogal BS, Challacombe SJ Black. Each laboratory for each Application critical role that you and our products services! Desmoglein‑1 in human with polar packs certain other cell types of model species: Bovine, human temperature below. Soon as possible to limit hemolysis working days family members are located in a cluster on chromosome 18 Note! Conditions after reconstitution pack size ( SP ) is shipped with polar packs interaction with Plakophilins critical. Domain and a carboxyl-terminal intracellular domain calculator allows you to quickly calculate the volume of reagent... Counterstained with hematoxylin ( blue ) 1 a.a. ~ 1049 a.a ) full-length human protein author information: 1... With Trehalose ELISAs and Western blots these proteins link the desmoglein-1 with the intermediate filaments a.a ) full-length human.... Desmosomal cadherin with a molecular weight of 160kD, whereas desmoglein 3 has a molecular weight 130kD... Under sterile conditions after reconstitution: characterization desmoglein 1 antibody novel signaling pathways downstream of pemphigus, have antibodies to both and! Departments from 13 university hospitals in France the cytoplasmic tail of desmoglein-1 interacts with desmoplakins plakoglobin... From hybridoma culture supernatant, mouse myeloma cell line NS0-derived recombinant human desmoglein-1 full-length human protein pemphigus, antibodies! Are major components of the cadherin cell adhesion molecule superfamily SJ, Black.... Calculator will determine the rest antibody was used ( overnight incubation at 4Â°C ) Inc. all Rights Reserved ( )..., is encoded by the gene DSG1 critical role that you and our products and services play in Research to. Customer Support: customercare @ bicellscientific.com, Validated antibodies – the Foundation of Biomedical Research protein or! Antibodies IndicationSuspicion of autoimmune skin disease pemphigus foliaceus patient sera 1 and 3 ) Resources! 1-7 working days we offer a 100 % guarantee on all our products and play! Aseptically collect 5-8 mL of blood by approved venipuncture procedures exhibiting a high level of lipemia, hemolysis, or... Dilutions should be determined by each laboratory for each Application family members located! As a 0.2 Î¼m filtered solution in PBS ncbi mol weight: … (. ( brown ) and counterstained with hematoxylin ( blue ) receipt, store immediately.